Application

This rabbit polyclonal Anti-SLP76, Alexa Fluor^® 488 Conjugate, Cat. No. ABF1215-AF488 is validated for use in Flow Cytometry for the detection of SLP76 (lymphocyte cytosolic protein 2).

Flow Cytometry Analysis: 1:400 dilution from a representative lot detected SLP76 in 4% paraformaldeyde-fixed, 0.2% Triton X-100-permeabilized THP-1 human monocytic cells.

The unconjugated antibody (Cat. No. ABF1215) is also available for Western blotting application.

General description

Lymphocyte cytosolic protein 2 (UniProt: Q13094; also known as Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76) is encoded by the gene LCP2 (Gene ID 3937) in human. SLP-76 is a cytoplasmic adaptor phosphoprotein that contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. Its amino terminus contains an acidic region that includes a PEST domain and several tyrosine residues that are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a SH2 domain at its carboxy terminal. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind and this complex stimulates NFAT and IL-2 gene activation after TCR engagement. SLP-76 is highly expressed in spleen, thymus, and peripheral blood leukocytes and T cells. It is expressed in B cell lines at lower levels and is not detected in fibroblast and neuroblastoma cell lines. SLP-76 is reported to play a positive role in promoting T cell development and activation as well as mast cell and platelet function. Overexpression of SLP-76 is shown to enhance IL-2 transcription after TCR signaling.

Immunogen

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human SLP76.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: A 1:400 diluution of this antibody detected SLP76 in 4% paraformaldeyde-fixed, 0.2% Triton X-100-permeabilized Jurkat human T lymphocytes.

Specificity

This rabbit polyclonal antibody Alexa Fluor^® 488 conjugate detected SLP76 (lymphocyte cytosolic protein 2) in Jurkat cells by flow cytometry.

Target description

60.2 kDa calculated.